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Nu Page Mops Sds Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page loading buffer  (TaKaRa)
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Sds Page Loading Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page running buffer  (Thermo Fisher)
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Thermo Fisher sds page running buffer
A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. <t>C)</t> <t>SDS-PAGE</t> and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.
Sds Page Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sds page running buffer/product/Thermo Fisher
Average 98 stars, based on 1 article reviews
sds page running buffer - by Bioz Stars, 2026-04
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sds page buffer  (Thermo Fisher)
99
Thermo Fisher sds page buffer
A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. <t>C)</t> <t>SDS-PAGE</t> and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.
Sds Page Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sds page buffer/product/Thermo Fisher
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quick fluorescent loading buffer  (Valiant Co Ltd)
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Valiant Co Ltd quick fluorescent loading buffer
A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. <t>C)</t> <t>SDS-PAGE</t> and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.
Quick Fluorescent Loading Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris glycine sds page sample buffer  (Thermo Fisher)
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Thermo Fisher tris glycine sds page sample buffer
A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. <t>C)</t> <t>SDS-PAGE</t> and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.
Tris Glycine Sds Page Sample Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nupage mops sds page running buffer  (Thermo Fisher)
96
Thermo Fisher nupage mops sds page running buffer
A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. <t>C)</t> <t>SDS-PAGE</t> and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.
Nupage Mops Sds Page Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nupage mops sds page running buffer/product/Thermo Fisher
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nupage mops sds page running buffer - by Bioz Stars, 2026-04
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A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. C) SDS-PAGE and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.

Journal: bioRxiv

Article Title: Rescue of pATOM36-depleted T. brucei by human MTCH1/2 reveals common features of protein insertases

doi: 10.64898/2026.02.13.705746

Figure Lengend Snippet: A) Schematic representation of the pATOM36-GFP construct used for overexpression and the TEV-digested pATOM36 obtained after the purification procedure. B) Immunofluorescence microscopy of pATOM36-GFP (green) in yeast, co-stained with MitoTracker (yellow) to visualize mitochondria. C) SDS-PAGE and immunoblot of non-digested pATOM36-GFP and TEV-digested pATOM36 following detergent solubilization and Ni-NTA purification. D) Schematic of the in vitro lipid-scrambling assay. pATOM36 is light blue, lipids gray, fluorescent NBD-PE yellow, and Triton X-100 red. E) Time-course fluorescence measurements of lipid scrambling in pATOM36 proteoliposomes (blue) versus empty liposomes (red) and CybB561 proteoliposomes (orange). Liposomal membrane integration of pATOM36 was further assessed by alkaline carbonate extraction, where reconstituted proteins sediment in the pellet (Pel) and non-reconstituted proteins remain in the supernatant (Sup), compared to total input (Inp) by SDS-PAGE and immunoblotting.

Article Snippet: Prior to transfer, gels were equilibrated 5 min in SDS-PAGE running buffer (25 mM Tris, 1 mM EDTA, 190 mM glycine, 0.05% (w/v) SDS) to facilitate protein transfer to PVDF membranes (Thermo Fisher Scientific) in 20 mM Tris, 150 mM glycine, 0.02% SDS, 20% methanol , .

Techniques: Construct, Over Expression, Purification, Immunofluorescence, Microscopy, Staining, SDS Page, Western Blot, In Vitro, Fluorescence, Liposomes, Membrane, Extraction